Try again. If the contract between the depositor and the bank states that two signatures are required (that means the bank agreed to the requirement, and it has no escape language in its contract), a missing signature is the legal equivalent to having no authorized signature.
Midnight deadline applies.
Now your decision is whether the customer is entitled to collect from you. That's a separate question.
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John S. Burnett
BankersOnline.com
Fighting for Compliance since 1976
Bankers' Threads User #8