When placing holds on funds, is it based on the 'total deposit' or each individual check if more than one check make up the deposit? For example: there are 2 checks. One for 3,000 and another one for 3,000. Would the amount of the hold (making $100 available) be $5,900 or $5,800?

$5900.00

Regg CC requires only that the first $100 of the aggregate total of local and non-local checks deposited during the day be made available. It's not a violation to be a little generous and give an extra $100 availability the next day, but you are not required to do so.